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Root number
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101717 |
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Semester
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FS2024 |
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Type of course
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Group Laboratory |
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Allocation to subject
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Biomedical Engineering |
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Type of exam
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not defined |
| Title |
Tissue Engineering - Practical Course |
| Description |
Auditors (Gasthörer) will not be admitted to this course.
Course Type: 2-week Laboratory Block Course
Module: Recommended Elective Courses / Major Module "Biomechanical Systems"
Selection criterion: This course will be limited to 3 groups à 2 people. 1 or 2 places are reserved for PhD students. Next in line are students with Major Module Biomechanical Systems, followed by other BME students. The secondary selection criterion for BME students is the grade of the exam in Biological Principles of Human Medicine or the depth of knowledge acquired in previous studies.
Exam registration is not required for this course.
Course materials are regularly posted on Ilias (www.ilias.unibe.ch).
The primary aim of this course is to become familiar with the basics of cell and tissue culture. The students will be introduced to basic laboratory techniques doing actual bench work. It is intended for biomedical engineers that are “beginners” of cell culture and tissue engineering. High weight will be given to 3D culture systems, such as hydrogels but also to the most commonly used protocols to characterize a culture system, such as proliferation, activity, and matrix production, e.g. DNA content, glycosaminoglycan content, cell viability and cell activity, respectively. Students will learn how to maintain cells in culture in 2D but also in 3D culture using hydrogels. Furthermore, students can get familiar with proper pipetting and preparation of culture media. These skills will be exercised within the framework of a 2-week experiment where primary cells will be harvested from tissue from the abattoir. Cells will be seeded in 3D carriers, e.g. alginate, agarose and these culture systems will be compared to the 2D monolayer. The handling of growth factors and real-time reverse transcriptase-PCR will be demonstrated. The theory and own results of the practical exercises are discussed between the experimental work. Guest lectures on cardiac tissue engineering by PD Dr Marie-Noëlle Giraud, and on lung regeneration by Prof. Barbara Rothen-Rutishauser, and by PD Dr. Amiq Gazdhar, and finally on endothelial cells by PD Dr. Allessandro Bertolo will be given. This year, we are proud to announce a short introduction on micro-gel delivery systems using hyaluronic acid/collagen type 2 cross-linked hydrogels by Parisa Torabi, visiting Ph D candidate student from Technical University of Teheran, Iran.
Prerequisites:
• Course(s): Biological Principles of Human Medicine, Tissue Engineering
Recommended Courses/Skills:
• Skill(s): pipetting, stoichiometry, “molecular thinking”
Required Material or Equipment:
• Second pair of closed slippers for the change between non-sterile and sterile laboratory
• A Laboratory coat will be provided
Course Policies and Classroom Rules of Conduct:
• Academic dishonesty, plagiarism, and any other kind of fraud will lead to the exclusion from the course.
• Attendance required
• Punctuality
• No radios, audio/cd player, earphones
• No food and beverages in the lab |
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ILIAS-Link (Learning resource for course)
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Registrations are transmitted from CTS to ILIAS (no admission in ILIAS possible).
ILIAS
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Link to another web site
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Further information for this course
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| Lecturers |
Prof. Dr.
Benjamin Gantenbein, Clinic of Orthopaedic Surgery ✉
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Yamina Baumgartner, Clinic of Orthopaedic Surgery ✉
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Paola Bermudez, Clinic of Orthopaedic Surgery ✉
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PD Dr.
Alessandro Bertolo, ARTORG Center - Master in Biomedical Engineering ✉
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Katherine Briana Crump, Clinic of Orthopaedic Surgery ✉
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PD Dr.med.
Amiq Gazdhar, Clinic of Pneumology and Allergology ✉
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Nicole Nyfeler, Masters in Biomedical Engineering ✉
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PD Dr.
Barbara Rothen-Rutishauser, Masters in Biomedical Engineering ✉
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Leon Max Schlagenhof, Clinic of Orthopaedic Surgery ✉
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Parisa Torabi Rahvar, Clinic of Orthopaedic Surgery ✉
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ECTS
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2 |
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Recognition as optional course possible
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No |
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Grading
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1 to 6 |
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| Dates |
Monday 5/2/2024 09:15-11:00
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Monday 5/2/2024 11:15-12:00
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Monday 5/2/2024 13:30-17:30
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Tuesday 6/2/2024 09:15-12:00
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Wednesday 7/2/2024 08:15-09:00
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Wednesday 7/2/2024 09:15-10:00
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Wednesday 7/2/2024 10:00-12:00
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Wednesday 7/2/2024 13:30-16:00
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Thursday 8/2/2024 09:15-10:00
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Thursday 8/2/2024 10:00-12:00
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Thursday 8/2/2024 13:30-15:00
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Thursday 8/2/2024 15:00-16:00
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Friday 9/2/2024 08:15-09:00
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Friday 9/2/2024 09:00-10:00
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Friday 9/2/2024 10:00-12:00
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Monday 12/2/2024 08:15-12:00
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Monday 12/2/2024 13:30-17:30
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Tuesday 13/2/2024 08:15-11:00
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Tuesday 13/2/2024 11:15-12:00
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Tuesday 13/2/2024 13:30-16:00
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Tuesday 13/2/2024 16:00-17:30
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Wednesday 14/2/2024 08:15-09:00
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Wednesday 14/2/2024 09:00-12:00
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Wednesday 14/2/2024 09:00-12:00
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Wednesday 14/2/2024 13:30-16:00
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Wednesday 14/2/2024 13:30-16:00
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Wednesday 14/2/2024 16:00-17:30
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Thursday 15/2/2024 08:15-12:00
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Thursday 15/2/2024 13:30-17:30
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Thursday 15/2/2024 13:30-17:30
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Friday 16/2/2024 09:00-10:00
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Friday 16/2/2024 10:00-12:00
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Rooms
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| Students please consult the detailed view for complete information on dates, rooms and planned podcasts. |
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