12284-HS2020-0-Workshop: Conventional fluorescence microscopy, laser scanning microscopy and digital image processing





Root number 12284
Semester HS2020
Type of course Workshop
Allocation to subject Microscopy
Type of exam not defined
Title Workshop: Conventional fluorescence microscopy, laser scanning microscopy and digital image processing
Description (To register contact the teacher: fabian.blank@dbmr.unibe.ch)

Teaching of basics

1. Sample preparation (theory)
a. The use of fixed samples (what is the ideal fixation method?)
b. Labelling of individual samples with immunofluorescence and fluorescence dyes
c. Mounting of samples (requirements for mounting media and coverslips etc.)

2. Microscopy (Practical)
a. Conventional Fluorescence Microscopy
b. Single-point confocal
c. Multi-point confocal
d. Live cell imaging

3. Image processing (Practical)
a. Visualize, format and process your data, employing FIJI
b. Optimizing fluorescence signal quality (deconvolution, Huygens Remote Manager)
c. Image processing and image presentation (IMARIS, Bitplane)
ILIAS-Link (Learning resource for course) Registrations are transmitted from CTS to ILIAS (no admission in ILIAS possible). ILIAS
Link to another web site
Lecturers PD Dr. phil. nat. Fabian BlankDepartment for BioMedical Research, Live Cell Imaging (LCI) 
Carlos Wotzkow AlvarezDepartment for BioMedical Research (DBMR) 
Selina Katja SteinerDepartment for BioMedical Research (DBMR) 
Dr. Yury BelyaevMicroscopy Imaging Center (MIC) 
Dr. Guillaume Robert WitzInstitute of Mathematics 
ECTS 1.5
Recognition as optional course possible Yes
Grading 1 to 6
 
Dates Monday 7/12/2020 08:15-17:00
Tuesday 8/12/2020 08:15-17:00
Wednesday 9/12/2020 08:15-17:00
 
Rooms
 
Students please consult the detailed view for complete information on dates, rooms and planned podcasts.