2807-HS2024-0-Lab course molecular biology





Root number 2807
Semester HS2024
Type of course Group Laboratory
Allocation to subject Cell Biology
Type of exam Written exam
Title Lab course molecular biology
Description Molecular Biology Internship 2024
The internship takes place for 11 weeks starting at the beginning of the semester, all day on Thursday and Friday. Work is done in groups of two. A detailed schedule with the course program will be available on ILIAS about two weeks before the semester starts. CHF 100.00 for the script for the lab course will be charged.
The practical course includes the following parts:
- Introduction and theory of the experiments that will be performed in the lab.
- Practical work in the lab
- Theory in bioinformatics with exercises (to be handed in weekly).

On the last day of the practical, the written exam will take place and the handwritten lab journal must be handed in. No separate registration is required for the written exam


Practice
• restriction digest
• agarose gel analysis
• preparation competent bacteria
• cloning with homologous recombination
• side specific mutagenesis with PCR
• transfection in mammalian cells
• staining and image analysis of mammalian cells
• protein extraction and Western blot
• RNA extraction and reverse transcription
• qPCR
• protein expression in bacteria
• purification of protein
Bioinformatics
• plasmid mapping
• primer design
• sequence analysis
• image analysis
• qPCR evaluation
ILIAS-Link (Learning resource for course) Registrations are transmitted from CTS to ILIAS (no admission in ILIAS possible). ILIAS
Link to another web site
Lecturers Prof. Dr. Olivier PertzInstitute of Cell Biology 
Prof. Dr. Peter MeisterInstitute of Cell Biology 
Andreas GruberInstitute of Cell Biology 
Judith Maria TrübInstitute of Cell Biology 
ECTS 8
Recognition as optional course possible Yes
Grading 1 to 6
 
Dates Thursday 09:15-10:15 Weekly
Friday 09:15-10:15 Weekly
Thursday 09:15-17:00 Weekly
Friday 09:15-17:00 Weekly
Thursday 19/9/2024 13:00-14:00
Thursday 26/9/2024 13:00-14:00
Thursday 7/11/2024 13:00-14:00
Friday 29/11/2024 09:00-11:30
 
Rooms Hörraum C161, Zellbiologie
 
Students please consult the detailed view for complete information on dates, rooms and planned podcasts.